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Spread Plate Technique Advantage And Disadvantage. A perfect spread plate technique will results visible and isolated colonies of bacteria that are evenly distributed in the plate and are countable. Firstly users will not be able to grow obligate anaerobes using this method. It cant be used to count bacteria that are sensitive to temperatures above 42ºC b. Loss of viability of heat-sensitive organisms coming into contact with hot agar.
It cant be used to count bacteria that are sensitive to temperatures above 42ºC. In this method the volume of sample not greater than 01 ml can be spread on the nutrient agar plate because it would. Facultative anaerobes wont grow using this technique. A disadvantage of the spread plate technique is that. Last updated on August 26th 2019 Spread plate technique is a method employed to plate a liquid sample for the purpose of isolating or counting the bacteria present in that sample. Embedded colonies are much smaller than those which happen to be on the surface.
Loss of viability of heat-sensitive organisms coming into contact with hot agar.
One major advantage of spread spectrum technique is less vulnerability to being intercepted and interfered within a sense it is more secured. Advantages of Pour Plate Technique. The advantages and disadvantages of spread plate method are highlighted next. Only a small inoculation volume can be used. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. Embedded colonies are much smaller than those which happen to be on the surface.