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Pcdna3 1 Myc His Invitrogen. Three untagged versions of pcDNA31 available separately each with a different selectable marker Geneticin Zeocin or Hygromycin are. Solutions for every step in your journey to discovery. Details of the multiple cloning sites for pcDNAŽ31myc-His- A B and C are shown on pages 5-7. PcDNA31-Myc-His RTN3A1 was a gift from Jimtong Horng Addgene plasmid 31087.
1 760 603 7200 F. V790-20 and V795-20 Version K 10 November 2010 28-0104. 5 Sequencing 1 Primer Sequence. HindIII 1 911. 4185 4586 4620 4687 4797 5039. PcDNA31Myc-His A pcDNA31Myc-HisA.
Kit Contents The contents for each kit are listed below.
Thr Arg Ala Ala Ala Thr Val Leu Asp Ile Cys Arg Ile Pro Pro His Trp Thr Ser Gly Ser Glu Leu Gly Thr Lys Leu Gly Pro Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Ser Ala Val Asp His His His His His His TATA T7 promoterpriming site Apa I Xba I Xho I Not I BstX I EcoR V EcoR I BamH I Asp718 I BstX I Kpn I Hind III Apa I myc epitope. Thr Arg Ala Ala Ala Thr Val Leu Asp Ile Cys Arg Ile Pro Pro His Trp Thr Ser Gly Ser Glu Leu Gly Thr Lys Leu Gly Pro Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Ser Ala Val Asp His His His His His His TATA T7 promoterpriming site Apa I Xba I Xho I Not I BstX I EcoR V EcoR I BamH I Asp718 I BstX I Kpn I Hind III Apa I myc epitope. The figure below summarizes the features of the pcDNAŽ31myc-His- vectors. The control plasmid pcDNA31myc-HislacZ is the pcDNA31myc-His C vector with a 32-kb fragment containing the -galactosidase gene cloned in frame with the C-terminal peptide see page 9. Upon receipt store vectors at 20C. Control plasmid pcDNA31myc-His-lacZ is the pcDNA31myc-His- A vector with a 32 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide see page 9.