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Inducible Mirna Expression Vector. Download Full PDF Package. The preferred inducible vector pLKO-puro-IPTG-3xLacO 399 KB PDF contains three lac operon sequences two in the U6 promoter and one 3 of the promoter affording both. Knockdown of the Pre-miRNA Processing Enzyme Drosha RNA interference RNAi is a powerful genetic tool for loss-of-function studies in mammalian cells and is also considered a potentially powerful therapeutic modality for the treatment of a variety of human diseases. VectorBuilder offers a comprehensive collection of 2nd and 3rd generation Tet inducible gene expression system reagents to achieve tetracycline-regulated gene expression.
The kit contains the pT-REx-DEST30 Gateway vector which after simple cloning and shuttling techniques produces a miR RNAi expression vector suitable for inducible knockdown. Cellutron has developed a novel inducible expression system with Cre-Loxp technology and provided the ability to regulate transgene expression. 16 rows Lentiviral plasmid for inducible expression of transgene of interest and EGFP. Download Full PDF Package. An additional feature of the system is that a bright red fluorescent protein marker mCherry is coexpressed along with your miRNA enabling you to verify miRNA expression in intact cells. The preferred inducible vector pLKO-puro-IPTG-3xLacO 399 KB PDF contains three lac operon sequences two in the U6 promoter and one 3 of the promoter affording both.
A Facile Lentiviral Vector System for Expression of Doxycycline-Inducible shRNAs.
Inducible Expression Vector System. Store the reagents at 20C. A Facile Lentiviral Vector System for Expression of Doxycycline-Inducible shRNAs. Expression of more than one engineered miR RNAi sequence on the same transcript allowing the knockdown of multiple genes simultaneously and the generation of synthetic phenotypes The new BLOCK-iT Inducible Pol II miR RNAi Expression Vector Kit with EmGFP provides the ability to regulate RNAi experiments. The pmR-mCherry and pmR-ZsGreen1 Vectors couple your miRNA expression cassette to a bright red or green Living Colors fluorescent reporter for miRNA expression you can see and select. The Mir-X Inducible miRNA System Red is a set of expression vectors that allows you to establish a Tet-regulated microRNA miRNA expression system in mammalian cells.